Nutrient Agar 100g
Add water and sterilize for a general-purpose nutrient agar base.
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Nutrient agar is the most commonly used nutrient media for the cultivation of microbes in petri dishes. It contains many nutrients needed for the growth of bacteria and fungi.
Check out this experiment on how to grow bacteria on homemade agar plates!
Nutrient agar is a light yellow powder. One gram of powder dissolved in 35 ml of boiling water will make enough general-purpose nutrient agar base for 7 standard 90 mm petri dishes in a thin layer. (5 mL of nutrient agar is just enough to cover one standard 90 mm petri dish.)
Instructions to prepare nutrient agar:
Dissolve 1 gram of nutrient agar powder in 35ml boiling water. Distilled water is preferred but tap water can be used. Sterilize by autoclaving at 121 degrees Celsius for 15 minutes. If no autoclave is available you can put the nutrient agar in the microwave for 15 to 20 seconds.
Sterilizing at home:
If you do not have access to an autoclave, a pressure cooker can be used to sterilize nutrient agar. In the absence of a pressure cooker, bacteria can still be grown on nutrient agar that is not completely sterile, however you run the risk of contamination from bacteria in the water/nutrient agar. In this instance it is best to boil the nutrient agar in a microwave for 15 to 20 seconds to kill as much bacteria as possible and to ensure the agar is fully dissolved.
Note: Keep in a dry, sealed container and store in a cool dark location away from bright light
What you get
Nutrient Agar laboratory grade powder, 100g.
WARNING: CHOKING HAZARD Small Parts. Not for children under 3 years.